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Phi29 DNA Polymerase

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产品名称: Phi29 DNA Polymerase
产品型号: E014
产品展商: ABM
产品文档: 无相关文档

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Phi29 DNA Polymerase


Phi29 DNA Polymerase  的详细介绍
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Description Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.
Application • Rolling circle amplification (RCA)
• Multiple displacement amplification (MDA)
• Whole genome amplification (WGA)
• DNA template preparation for sequencing
• Protein-primed DNA amplification
Form Enzyme supplied with 5X Reaction Buffer
Image
Concentration 10 U/μl
Storage Store all components at -20°C.
Storage Buffer 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol.
Notes One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.

This product is distributed for laboratory research only.
Caution: Not for diagnostic use .
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