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T4 DNA Polymerase

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产品名称: T4 DNA Polymerase
产品型号: E005
产品展商: ABM
产品文档: 无相关文档

简单介绍

T4 DNA Polymerase


T4 DNA Polymerase  的详细介绍
Print Version
Description T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
Application
  • Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs
  • High fidelity due to strong 3’→5’ exonuclease activity
  • Synthesis of labeled DNA probes by the replacement reaction
  • Site-specific mutagenesis via primer extension from oligonucleotides
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    Form Enzyme supplied with 10X Reaction Buffer
    Image
    Concentration 5 U/μl
    Storage Store all components at -20°C.
    Storage Buffer 100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.
    Notes One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.

    This product is distributed for laboratory research only.
    Caution: Not for diagnostic use.
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