产品资料

dCas9 C-terminal Cloning Vector

如果您对该产品感兴趣的话,可以
产品名称: dCas9 C-terminal Cloning Vector
产品型号: K014
产品展商: ABM
产品文档: 无相关文档

简单介绍

dCas9 C-terminal Cloning Vector


dCas9 C-terminal Cloning Vector  的详细介绍
Print Version
Description This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal.
Product Application
  • Direct non-viral plasmid transfection for immediate expression
  • Package into Lentiviral particles for high efficiency transduction and stably integrated expression
    		•
    Species N/A
    Vector pLenti-EF1a-dCas9-Cloning
    Bacterial Selection Kanamycin
    Format Plasmid
    Appearance Liquid
    Storage -20°C or below
    Shelf Life 1 year (when at -20°C or below in a non-frost free freezer)
    Shipping Shipped at ambient temperature
    Additional Materials - CRISPR Genomic Cleavage Detection Kit (G932)
    - sgRNA CRISPR Lentivector Control (K018)
    Quality Control Restriction Enzyme Digest and Sequencing
    Caution This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
    Note abm's sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant).
    Disclaimer 1. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials.
    产品留言
    标题
    联系人
    联系电话
    内容
    验证码
    点击换一张
    注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
    2.如有必要,请您留下您的详细联系方式!

    沪公网安备 31011202007337号