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Gateway BP Cloning Kit

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产品名称: Gateway BP Cloning Kit
产品型号: E082
产品展商: ABM
产品文档: 无相关文档

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Gateway BP Cloning Kit


Gateway BP Cloning Kit  的详细介绍
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Description The Gateway cloning system from abm is a flexible cloning method that allows for easy subcloning of one Entry Clone into a wide variety of destination vectors. Gateway technology makes it easy to study your gene of interest using many different promoters and tags. It eliminates the need for restriction enzyme digests or ligations by harnessing the natural ability of phage λ to integrate its DNA into the E. coli chromosome at specific attachment (att) sites. Gateway reaction recombinations are conservative, directional, and always maintain reading frame. The Gateway system uses both positive selection (antibiotic resistance) and negative selection (toxin-producing ccdB gene) during transformation, ensuring a high proportion of successful recombinants.

Use abm’s Gateway BP Cloning Kit (E082/E083) with a pDONR vector and your gene flanked by attB sites to create your own Entry Clone. It’s then quick and easy to insert your gene of interest into any destination vector using our Gateway LR Cloning Kit (E084/E085). These kits contain the enzyme mixes to perform.
Application
  • Easily clone your gene from one Entry Clone into many expression systems with a variety of promoters and tags.
  • No dependence on restriction enzyme digests or ligations.
  • Maintains reading frame and orientation.
  • Fast and Easy.
  • Less expensive than other commercially available kits.
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