[qPCR/Electrophoresis]dsGreen Gel Staining Solution, 10000×[lumiprobe]
dsGreen Gel Staining Solution, 10000×
详细描述
订购货号产品名称及规格价格(¥)
20010dsGreen Gel Staining Solution, 10000×, 1 mL2340.00
SYBR Green I是一种敏感的dsDNA结合染料,可以用于日常琼脂糖和聚丙烯酰胺凝胶的DNA检测。
不同于Ethidium,SYBR Green I对双链DNA有高度选择性,更低的毒性,更高的灵敏度。
溴化乙锭(Ethidium Bromide)和SYBR Green I的对比
FeatureEthidium BromideSYBR Green I
FluorescenceRed (615 nm)Green (524 nm)
Excitation maximum302 nm454 nm
Excitation light sourceUV onlyBlue light or UV
Sensitivity2 ng / band(dsDNA)
100 ng / band (RNA)0.08 ng / band (dsDNA)
1-2 ng / band (oligonucleotides)
Health hazardHighLow
推荐手册:
SYBR Green I DNA凝胶染色
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
General properties
Appearance: orange solution
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Spectral properties
Excitation maximum, nm: 454
Extinction coefficient at excitation maximum, Lmol-1cm-1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0.8
[qPCR/Electrophoresis]dsGreen Gel Staining Solution, 10000×[lumiprobe]