Description Viva SybrGreen Nucleic Acid Stain is one of a kind novel generation of fluorescent nucleic acid gel stain used to detect double-stranded DNA in agarose and polyacrylamide gels without requiring a destaining step. The detection limit using Viva SybrGreen Nucleic Acid Stain is as low as 60pg doublestranded DNA at 300nm transillumination and as little as 20pg double-stranded DNA can be detected at 254nm illumination. Single-stranded DNA and RNA can be detected at approximately 100pg to 300pg per well.
Viva SybrGreen Nucleic Acid Stain is designed to replace the highly toxic ethidium bromide (EtBr). The sensitivity is 25 times greater than ethidium bromide. Viva SybrGreen Nucleic Acid Stain is nontoxic and more sensitive than EtBr.
Features Safer The dye is noncytotoxic & nonmutagenic shown by Ames tests. Higher sensitivity 5-10X more sensitive than EtBr under UV and 8-20X more sensitive than EtBr in visible light. Extremely stable Stable at room temperature for long-term storage. Stable to be microwaved or being heated. The working solution is stable at room temperature when kept in dark. Wide application Suitable to stain dsDNA, ssDNA and RNA. Suitable to use in agarose gel or polyacrylamide gel. Compatible with downstream applications, such as gel recovery & cloning. Easy staining protocols Easy precast gel staining, pre-staining and post-staining procedures. Compatible with most imaging system Gel can be viewed with standard UV transilluminator, visible light gel reader or other gel imaging system.
Storage Buffer Shipped at ambient temperature Stored at 2-8℃ or -20℃
Recommended Equipment Required But Not Supplied:
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Viva SybrGreen Nucleic Acid Stain (10000X in DMSO)
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