IP antibody use: 5ug VSV-G Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with VSV-G-tag fusion protein 3) IP (untransfted 293+anti-VSV-G mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse
IF analysis of 293T cells transfected with a VSV-G-tagged protein,1:2000 dilution (blue DAPI, red anti-VSV-G)
1ug VSV-G fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
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