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dsGreen Gel Staining Solution, 10000×

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产品名称: dsGreen Gel Staining Solution, 10000×
产品型号: A0010 10010 20010
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简单介绍

dsGreen Gel Staining Solution, 10000× A0010 10010 20010


dsGreen Gel Staining Solution, 10000×  的详细介绍

dsGreen Gel Staining Solution, 10000×

PCR and qPCR
Cat. # Quantity Price Lead time
A0010 50 uL  29.00$ in stock
 
10010 0.5 mL  100.00$ in stock
 
20010 1 mL  180.00$ in stock
 
Bulk inquiry 

dsGreen, an equivalent of SYBR® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

Comparison between ethidium bromide and dsGreen

Feature Ethidium Bromide dsGreen
Fluorescence Red (615 nm) Green (524 nm)
Excitation maximum 302 nm 454 nm
Excitation light source UV only Blue light or UV
Sensitivity 2 ng / band (dsDNA)
100 ng / band (RNA)
0.08 ng / band (dsDNA)
1–2 ng / band (oligonucleotides)
Health hazard High Low

Note: Fluorescence properties of SYBR Green I bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

dsGreen Gel Staining Solution, 10000×
dsGreen Gel Staining Solution, 10000×
dsGreen Gel Staining Solution, 10000×
dsGreen I structure
Download in 
ChemDraw format

Recommended protocol

DNA Staining in Gels with dsGreen or SYBR Green I

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General properties

Appearance: orange solution
Quality control: NMR 1H, HPLC-MS (95%), functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 454
ε, L?mol?1?cm?1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0.8

Product citations

  1. Zietzer, A.; Buschmann, E.E.; Janke, D.; Li, L.; Brix, M.; Meyborg, H.; Stawowy, P.; Jungk, C.; Buschmann, I.; Hillmeister, P. Acute Physical Exercise and Long-Term Individual Shear Rate Therapy Increase Telomerase Activity in Human Peripheral Blood Mononuclear Cells. Acta Physiologica, 2017, 220(2), 251–262. doi: 10.1111/apha.12820
  2. Diaz-Romero, J.; Kürsener, S.; Kohl, S.; Nesic, D. S100B+A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. Journal of Cellular Physiology, 2017, 232(6), 1559–1570. doi:10.1002/jcp.25682
  3. Richter-Heitmann, T.; Eickhorst, T.; Knauth, S.; Friedrich, M.W.; Schmidt, H. Evaluation of Strategies to Separate Root-Associated Microbial Communities: A Crucial Choice in Rhizobiome Research. Frontiers in Microbiology, 2016, 7, 773. doi:10.3389/fmicb.2016.00773
  4. Jain, D.; Siede, W. Rad5 Template Switch Pathway of DNA Damage Tolerance Determines Synergism between Cisplatin and NSC109268 in Saccharomyces cerevisiae. PLoS ONE, 2013, 8(10), e77666. doi: 10.1371/journal.pone.0077666
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