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Anti-Exendin-4 ABS 012-35

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产品名称: Anti-Exendin-4 ABS 012-35
产品型号: ABS 012-35
产品展商: Bioporto
产品文档: 无相关文档

简单介绍

Anti-Exendin-4


Anti-Exendin-4 ABS 012-35  的详细介绍
Cat. No.
ABS 012-35B
Product name
Anti-Exendin-4
Description

Mouse monoclonal antibody, biotinylated

Specificity

Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.

Immunogen

Synthetic exendin-4 

Application
ELISA
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2740
Clone number
35
Epitope

The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exendin (9-39)amide.

Content

50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Exendin-4 is a 39 amino acid peptide found in venom from the Gila monster Heloderma suspectum. It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion.

Application 1

ELISA
ABS 012-35 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay (1).

By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. ABS 033-10 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.

1. Murphy RE, Kinhikar AG, Shields MJ, Rosario JD, Preston R, Levin N, Ward GH (2010) Combined use of immunoassay and two-dimensional liquid chromatography mass spectrometry for the detection and identification of metabolites from biotherapeutic pharmacokinetic samples. Journal of Pharmaceutical and Biomedical Analysis 53:221-227.

20547023
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