This immunogenicity assay measures the presence of human IgM in serum or plasma that bind to immobilized PEG using the direct ELISA technique. The supplied stretavidin precoated 96 well microplate is incubated with biotinylated PEG (5KDa). After washing, quality control and test samples are pipetted into the appropriate wells. Anti-PEG antibodies bind the immobilized PEG. After washing, detection antibody (anti human IgM Peroxidase) is added. Any unbound antibody enzyme reagent is removed with a final wash and a substrate solution is added to the wells for color development. Color development is proportional to the amount of anti-PEG IgM.
Peroxidase / OD450
Stable at -20°C for 1 year
Research Use Only. Follow instructions.
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
62.5ng/ml
Coated microtiter plate, 96 wells QC samples - 6x250ul 10X assay/wash buffer - 50ml 500X biotinylated PEG(5KDa) - 50ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
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