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Etanercept (Enbrel®) Pharmacokinetic ELISA

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产品名称: Etanercept (Enbrel®) Pharmacokinetic ELISA
产品型号: EL-1611-051
产品展商: AffinityImmuno
产品文档: 无相关文档

简单介绍

Etanercept (Enbrel®) Pharmacokinetic ELISA Catalog EL-1611-051 $599.99


Etanercept (Enbrel®) Pharmacokinetic ELISA  的详细介绍
INTRODUCTION
Etanercept (trade name Enbrel®) is a protein drug used to treat autoimmune diseases by adsorbing tumor necrosis factor (TNF; a soluble inflammatory cytokine). Etanercept is a fusion protein produced through expression of recombinant DNA by linking the extracellular ligand-binding portion of TNFRSF1B to the Fc component of human immunoglobulin G1 (IgG1). It reduces the effect of naturally present TNF, functioning as a decoy receptor that binds to TNF.
Etanercept is indicated for the treatment of moderate to severe rheumatoid arthritis (RA), psoriatic arthritis, ankylosing spondylitis, and moderately to severely active polyarticular juvenile idiopathic arthritis. Serum concentration of ENBREL® may predict some clinical outcome during maintenance therapy.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Capture antibody is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Etanercept present in biological matrices is bound by the immobilized anti-Etanercept antibody. After washing away any unbound substances, enzyme linked anti-Etanercept antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Etanercept present in test samples. The color development is stopped and the intensity of the color is measured.

Etanercept (Enbrel®) Pharmacokinetic ELISA 
Catalog EL-1611-051


Product Properties

DETECTION METHOD

Peroxidase / OD450 

STORAGE AND STABILITY

Stable at -20°C for 1 year  

SAFETY WARNINGS AND PRECAUTIONS

Research Use Only. Follow instructions.  

PRECISION

Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. 

DETECTION LIMIT

1.5ng/ml 

EACH KIT INCLUDES:

Coated microtiter plate, 96 wells 
Calibrator diluent. - 1.8ml 
Calibrator (500 ug/mL) - 12ul 
10X wash buffer - 25ml 
Assay buffer - 50ml 
1000X detection reagent - 17ul 
TMB - 12ml 
TMB stop solution - 12ml 
Plate sealers - 3  

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