pTG19-T Cloning Vector

Description
The pTG19-T vector is designed for rapid and efficient cloning of PCR products with 3’dA overhangs. The linearized pTG19-T vector with 3’-dT overhangs prevent vector recircularization, therefore resulting in high percentage of recombinant clones and low background.

Features

• Convenient – ready-to-use linearized 3’dT overhang pTG19-T vector.
• Efficient –more than 80% of the recombinant clones contain the target DNA
• Rapid clone selection:
  -lacZ gene for blue/white selection.
  -M13 primer sites for PCR screening and sequencing.
  -BamHI restriction enzyme can be used to release the insert from the pTG19-T vector.

Quality Control

• More than 80% clones are white with control insert.
• More than 85% of white clones are positively by restriction endonuclease digestion.
• The 3’dT overhangs for every batch vectors is confirmed by sequencing of five recombinant clones.

Kit Components

• pTG19-T vector
• Control insert

Storage & Stability

• All components are stable at -20°C for one year if properly stored.
• To avoid frequent feeze-thaw cycles, keeping small aliquots at -20°C is recommended.

Ordering Information

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Manual
pTG19-T Cloning Vector

Publication
This Product Has Been Used In: 
Abbaszadeh-Goudarzi, K., Abbaszadeh-Goudarzi, G., Khorramizadeh, M.R., Bouzari, S., Rezaie, S., Davari, M., Siadat, S.D., Teimoori-Toolabi, L. (2017). HMW1555-914, HMW2553-916, and Hia585-705 as Subunit Vaccine Candidates of Nontypeable Haemophilus influenzae Induce Specific Antibody Responses with Bactericidal Activity in Balb/c, . Journal of Microbiology, Vol, 11, No. 1 (2017). 
Amini, S., Maali-Amiri, R., Mohammadi, R., Kazemi-Shahandashti,S.S. (2017) cDNA-AFLP analysis of transcripts induced in chickpea plants by TiO2 nanoparticles during cold stress. Plant Physiology and Biochemistry. 111. Pp39-49
Ayudhaya P.T.N., Muangmai N., Banjongsat N., Singchat W., Janekitkarn S., Peyachoknagul S., Srikulnath K. (2017), Unveiling cryptic diversity of the anemonefish genera Amphiprion and Premnas (Perciformes: Pomacentridae) in Thailand with mitochondrial DNA barcodes, In Agriculture and Natural Resources, Volume 51, Issue 3, Pages 198-205
Busayapongchai, P., Siri, S.(2017). Sensitive detection of estradiol based on ligand binding domain of estrogen receptor and gold nanoparticles. Analytical biochemistry. 518, pp.60-68.
Suntronpong, A., Thapana, W., Twilprawat, P., Prakhongcheep, O., Somyong, S., Muangmai, N., Peyachoknagul, S., Srikulnath, K. (2017). Karyological characterization and identification of four repetitive element groups (the 18S – 28S rRNA gene, telomeric sequences, microsatellite repeat motifs, Rex retroelements) of the Asian swamp eel (Monopterus albus), Comparative Cytogenetics, Vol. 11, No. 3, 435-462 (2017). 
Kohnehrouz, B.B., & Nayeri, S. (2016) Design, Cloning and In silico Analysis of Efficient siRNA-inducing Casette for Silencing Wheat γ-gliadins Jordan Journal of Biological Sciences, 9(1), p.35-40. 
Laopichienpong, N., Muangmai, N., Supikamolseni, A.,Twilprawat, P., Chanhome L., Suntrarachun, S., Peyachoknagul, S., Srikulnath, K. (2016). Assessment of snake DNA barcodes based on mitochondrial COI and Cytb genes revealed multiple putative cryptic species in Thailand. . Gene. 594(2).pp.238-247.
Rasoulinejad, S., Gargari, S.L.M. (2016) ) Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates. Journal of Biotechnology. 231. Pp.46-54 
Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38 
Meidaninikkjeh, S., Vaziri,F., Siadat, S.D. (2015) Cloning of conserved regions of nontypeable Haemophilus influenzae hmw1 core binding domain. International Journal of Molecular and Clinical Microbiology.5(1) pp.510-515 
Nasanit, R., et al (2015) Assesment of Epiphytic Yeast Diversity in Rice (Oryza sativa) Phyllospehere in Thailand by a Culture-independent Approach Antonie van Leeuwenhoek.Springer. 107(6), p.1475-1490
Valadan, R., Amjadi, O., Tehrani, M., Rafiei, A., Hedayatizadeh-Omran, Alizadeh-Navaei, R. (2015) Pseudogene-free amplification of HPRT1 in quantitative reverse transcriptase polymerase chain reaction. Analytical biochemistry. 485. Pp.46-48
Valadan, R., Hedayatizadeh-Omran, A., Alhosseini-Abayazani, M.N., Amjadi, O., Rafiei, A., Tehrani, M., Alizadeh-Navaei, R., (2015) Data supporting the design and evaluation of a universal primer pair for pseudogene-free amplification of HPRT1 in real-time PCR. Data in brief. 4. Pp.384-389. 
Babaei, S., Talebi, M., Bahar, M. (2014) Developing an SCAR and ITS reliable multiplex PCR-based assay for safflower *****erant detection in saffron samples. Food Control. 34. Pp..323-328