Pfu DNA Polymerase (Pyrococcus furiosus)

Description
Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperatures synthesis of DNA. Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg²⁺. It exhibits the 3' to 5' proofreading activity.

Features

• Ultra pure recombinant protein allows amplification up to 8kb.
• 5X ViBuffer Pfu provided for both short and long amplification.

Unit Definition
1u is defined as the amount of enzymes that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl₂ , 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100 μg/ml BSA and enhancer in a final volume of 50 μl.

Supplied With

• 50mM MgCl₂
• 5X ViBuffer Pfu

Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer
50mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.1% Tween™ 20, 0.1% Nonidet- P40, 0.1mM EDTA, 1mM DTT, and 50% glycerol. Store at -20°C.

Amplification of 1.5kb DNA fragment using Pfu DNA Polymerase with 10X ViBuffer A (Lane A1-A4) and 5X ViBuffer Pfu (Lane B1-B4).

1. PCR product using 2U of Pfu DNA Polymerase
2. PCR product using 1U of Pfu DNA Polymerase
3. PCR product using 0.5U of Pfu DNA Polymerase
4. PCR product using 0.25U of Pfu DNA Polymerase

Amplification of 1.5kb DNA fragment from Lambda DNA using Pfu DNA Polymerase with 10X ViBuffer S (Lane A1-A4) and 5X ViBuffer Pfu (Lane B1-B4).

1. PCR product using 2U of Pfu DNA Polymerase
2. PCR product using 1U of Pfu DNA Polymerase
3. PCR product using 0.5U of Pfu DNA Polymerase

Ordering Information

Download
Manual

Pfu DNA Polymerase

Comparison Test Report

Pfu DNA Polymerase

Publication
This Product Has Been Used In:
Sabouri, F., Marandi, M.V., Bashashati,M. (2017). Characterization of a novel VIIl sub-genotype of Newcastle disease virus circulating in Iran, Avian Pathology, Vol. 47, No. 1, 90-99 (2017).
Dalimi, A., Nasiri, V. (2016). Immunization with KMP11-NTGP96-GFP Fusion of Leishmania major Induced Th1 Platform Immune Response in Susceptible BALB/c mice, Jundishapur Journal of Microbiology, Vol. 9, No. 12 (2016)
Nasiri, V., Dalimi, A., Ghaffarifar, F., Bolhassani, A (2016). Immunogenicity and Efficacy of Live L. tarentolae Expressing KMP11-NTGP96-GFP Fusion as a Vaccine Candidate against Experimental Visceral Leishmaniasis Caused by J L. infantum, Iranian Journal of Parasitology, Vol. 11, No. 2 (2016).
Ashrafi, M., Farsi, M., Mirshamsi, A., Parvandi, M. (2015). Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008, International Journal of Horticultural Science and Technology,Vol. 2, No. 1, 33-41 (2015).
Dastsooz, H., Dehghani, S.M., Imanieh, M.H., Haghighat, M., Moini, M., Fardaei, M. (2013) A new ATP7B gene mutation with severe condition in two unrelated Iranian families with Wilson disease. Gene. 514(1) Pp.48-53
Dastsooz, H. et al. (2013) DNA Sequence Fragment Containing C to A Mutation as a Convenient Mutation Standard for DHPLC Analysis Iran J Basic Med Sci.; 16(8): 946–949
Özavci, V., Kirkan, Ş. (2013). Detection of Capnocytophaga canimorsus and Capnocytophaga cynodegmi by Cultural and Molecular Methods in Dogs in Western Turkey, of the Faculty of Veterinary Medicine, Kafkas University, Vol. 19, No. 5, 875-880 (2013).